Aerobic H2 production related to formate metabolism in white-rot fungi

Biohydrogen is mainly produced by anaerobic bacteria, fungi, and algae under conditions. In higher eukaryotes, it thought that molecular hydrogen (H 2 ) functions as a signaling molecule for physiological processes such stress responses. Here, demonstrated white-rot fungi produce H during wood decay. The fungus Trametes versicolor produces from aerobic conditions, production completely suppressed hypoxic Additionally, oxalate formate supplementation of the culture increased level evolution. RNA-seq analyses revealed T. TCA/glyoxylate cycle was down-regulated, conversely, genes encoding metabolism enzymes were up-regulated. Although involvement in gene annotated an iron hydrogenase uncertain, results organic acid supplementation, expression, self-recombination experiments strongly suggest plays role mechanism this fungus. It expected novel finding biomass will open new fields biohydrogen research.